Product Description

[KO Validated] MSH2 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS78188-50, BS78188-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P43246

Host: Rabbit

Reactivity: Human, Rat

Applications: WB, IF/ICC, IP,

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200 | IP,1:50 - 1:200

Background: This locus is frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). When cloned, it was discovered to be a human homolog of the E. coli mismatch repair gene mutS, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC. Two transcript variants encoding different isoforms have been found for this gene.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Bioworld Molecular Weight: 105KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of A-549, using MSH2 Rabbit pAb antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s., Western blot analysis of extracts from wild type and MSH2 Rabbit pAb knockout HeLa cells, using MSH2 Rabbit pAb antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s., Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug MSH2 antibody. Western blot was performed from the immunoprecipitate using MSH2 antibody at a dilution of 1:3000.

Alternative Name: MSH2; COCA1; FCC1; HNPCC; HNPCC1; LCFS2; mutS homolog 2

Immunogen: Recombinant fusion protein of human MSH2(NP_000242.1).

Conjugate: Unconjugated

Modification: Unmodified