[KO Validated] NG2/CSPG4 Polyclonal Antibody-BS80376

[KO Validated] NG2/CSPG4 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS80376-50, BS80376-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q6UVK1

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB,

All Applications: WB,1:500 - 1:2000

Background: A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Bioworld Molecular Weight: 251KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of various lysates, using NG2/CSPG4 Rabbit pAb antibody at 1:630 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s., Western blot analysis of extracts from wild type and NG2/CSPG4 Rabbit pAb knockout HeLa cells, using NG2/CSPG4 Rabbit pAb antibody at 1:630 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

Alternative Name: CSPG4; HMW-MAA; MCSP; MCSPG; MEL-CSPG; MSK16; NG2

Immunogen: Recombinant fusion protein of human NG2/CSPG4(NP_001888.2).

Conjugate: Unconjugated

Modification: Unmodified