[KO Validated] PDCD6 Polyclonal Antibody-BS8354

[KO Validated] PDCD6 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS8354-50, BS8354-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O75340

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IP,

All Applications: WB,1:500 - 1:2000 | IP,1:50 - 1:200

Background: This gene encodes a calcium-binding protein belonging to the penta-EF-hand protein family. Calcium binding is important for homodimerization and for conformational changes required for binding to other protein partners. This gene product participates in T cell receptor-, Fas-, and glucocorticoid-induced programmed cell death. In mice deficient for this gene product, however, apoptosis was not blocked suggesting this gene product is functionally redundant. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is also located on the short arm of chromosome 5.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 16KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts from wild type and PDCD6 knockout 293T cells, using PDCD6 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s., Immunoprecipitation analysis of 200ug extracts of HepG2 cells, using 3 ug PDCD6 antibody. Western blot was performed from the immunoprecipitate using PDCD6 antibody at a dilution of 1:1000.

Alternative Name: PDCD6; ALG-2; ALG2; PEF1B

Immunogen: Recombinant fusion protein of human PDCD6(NP_037364.1).

Conjugate: Unconjugated

Modification: Unmodified