[KO Validated] PHD2/EGLN1 Rabbit pAb - A14557

[KO Validated] PHD2/EGLN1 Rabbit pAb

Sizes: 20μL, 100μL

Catalogue Numbers: A14557-20, A14557-100

Citations, Manuals and MSDS Available upon request.

Background: The protein encoded by this gene catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. HIF is a transcriptional complex that plays a central role in mammalian oxygen homeostasis. This protein functions as a cellular oxygen sensor, and under normal oxygen concentration, modification by prolyl hydroxylation is a key regulatory event that targets HIF subunits for proteasomal destruction via the von Hippel-Lindau ubiquitylation complex. Mutations in this gene are associated with erythrocytosis familial type 3 (ECYT3).

Category: Polyclonal Antibodies

Applications: WB, IHC-P, IF/ICC, ELISA

Cross-reactivity: Human, Mouse, Rat

Protein Weight: 46kDa

Observed Molecular Weight: 50kDa

Immunogen: Synthetic peptide

Species: Human

GeneID: 54583

SWISS: Q9GZT9

Alternate Names: HPH2; PHD2; SM20; ECYT3; HALAH; HPH-2; HIFPH2; ZMYND6; C1orf12; HIF-PH2; N1

Source: Rabbit

Isotype: IgG

Purity: Affinity purification

Storage: Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis),  pH 7.3.

Recommended Dilutions: WB, 1:500 - 1:1000 IHC-P, 1:50 - 1:200 IF/ICC, 1:50 - 1:200 ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Research Areas: Epigenetics Nuclear Signaling, Cancer, Invasion and Metastasis, Endocrine Metabolism, Cardiovascular, Angiogenesis, Hypoxia.

NCBI Alias: EGLN1

Research Use Only