Product Description

[KO Validated] RhoGDI Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS74758-50, BS74758-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P52565

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IF/ICC

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200

Background: This gene encodes a protein that plays a key role in the regulation of signaling through Rho GTPases. The encoded protein inhibits the disassociation of Rho family members from GDP (guanine diphosphate), thereby maintaining these factors in an inactive state. Activity of this protein is important in a variety of cellular processes, and expression of this gene may be altered in tumors. Mutations in this gene have been found in individuals with nephrotic syndrome, type 8. Alternate splicing results in multiple transcript variants.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Bioworld Molecular Weight: 25kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts from normal and RhoGDI knockout 293T cells, using RhoGDI antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s., Immunofluorescence analysis of HeLa cells using RhoGDI antibody. Blue: DAPI for nuclear staining.

Alternative Name: ARHGDIA; GDIA1; HEL-S-47e; NPHS8; RHOGDI; RHOGDI-1

Immunogen: Recombinant fusion protein of human RhoGDI(NP_001172006.1).

Conjugate: Unconjugated

Modification: Unmodified