(KO Validated) Smad1 Polyclonal Antibody
Sizes: 60μL, 120μL, 200μL
Catalogue Numbers: E-AB-92425-60, E-AB-92425-120, E-AB-92425-200
Citations, Manuals and MSDS Available upon request.
Abbreviation: Smad1
Target Synonym: SMAD1; BSP-1; BSP1; JV4-1; JV41; MADH1; MADR1; SMAD family member 1
Research Areas: Cancer, Epigenetics and Nuclear Signaling, Signal Transduction, Stem Cells
Conjugation: Unconjugated
Host: Rabbit
Species Reactivity: Human, Mouse, Rat
Application: WB, IHC, IF
Isotype: IgG
Clonality: Polyclonal
UNIProt ID: Q15797
Background: The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed.
Concentration: 1mg/mL
Immunogen: Recombinant fusion protein of human Smad1
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Purification Method: Affinity purification
Dilution: WB 1:500-1:2000, IHC 1:50-1:200, IF 1:50-1:200
Calculated MW: 15kDa/52kDa
Observed MW: 60KDa
Storage: -20°C/One year. Avoid freeze / thaw cycles.
Shipping: Ice bag
Research Use Only