KSR (R386) Polyclonal Antibody-BS1707

KSR (R386) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS1707-50, BS1707-100

Sizes: 50µl, 100µl

Swiss-Prot: Q8IVT5

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

Background: Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk) and Raf-1. Raf-1 has intrinsic kinase activity towards serine/threonine residues and is widely expressed in many tissue types and cell lines. Raf-1 activation is dependent on the small molecular weight GTPase Ras, but the means by which this activation occurs is poorly understood. Two proteins putatively involved in this process are Ksr-1 and Tak1. Ksr-1 (kinase suppressor of Ras) is a novel Raf-related protein kinase whose function is required for Ras signal transduction. Whether Ksr-1 lies directly downstream of Ras or acts in a parallel pathway is not yet known. Tak1 (TGFb-activated kinase) has been shown to participate in the activation of the MAP kinase family in response to TGFb stimulation.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: KSR (R386) polyclonal antibody detects endogenous levels of KSR protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of KSR (R386) pAb at 1:500 dilution
Lane 1: SGC7901 whole cell lysate (40ug)
Lane 2: Hela whole cell lysate (40ug)
Lane 3: BV2 whole cell lysate (40ug)
Lane 4: C6 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of KSR (R386) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.

Bioworld Molecular Weight: ~ 115 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Kinase suppressor of Ras 1; KSR1; KSR

Immunogen: Synthetic peptide, corresponding to amino acids 350-402 of Human KSR.

Conjugate: Unconjugated

Modification: Unmodified