Ksr2 (I694) Polyclonal Antibody-BS2321

Ksr2 (I694) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS2321-50, BS2321-100

Sizes: 50µl, 100µl

Swiss-Prot: Q6VAB6

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

Background: Kinase suppressor of Ras (Ksr) and MAP kinase kinase kinase (MEKK3) are integral members of the MAP kinase pathway. Ksr is a conserved protein that positively regulates Ras signaling and may function as a scaffold for Raf, MEK and ERK. There are two types of Ksr proteins: Ksr-1 and Ksr-2. Ksr-2 plays a key role in Ras-mediated signaling during germline meiotic progression and functions redundantly with Ksr-1 during the development of the excretory system pathway, hermaphrodite vulva, and male spicules. Ksr-2 also functions as a negative regulator of the MEKK3-mediated activation of the MAP kinase pathways (specifically ERK and JNK) and of the NF˚B pathways, and it simultaneously inhibits MEKK3-mediated Il-8 production.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Ksr2 (I694) polyclonal antibody detects endogenous levels of Ksr2 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Ksr2 (I694) polyclonal antibody at 1:500 dilution
Lane 1: HEK293T whole cell lysate (40ug)
Lane 2: A549 whole cell lysate (40ug)
Lane 3: AML-12 whole cell lysate (40ug)
Lane 4: C6 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of Ksr2 (I694) pAb in paraffin-embedded human brain tissue.
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Bioworld Molecular Weight: ~ 108 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: KSR2; Kinase suppressor of Ras 2

Immunogen: Synthetic peptide, corresponding to amino acids 662-724 of Human Ksr2.

Conjugate: Unconjugated

Modification: Unmodified