Ku-70/XRCC6 (7B1) Monoclonal Antibody-MB0185

Ku-70/XRCC6 (7B1) Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB0185-50, MB0185-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P12956

Host: Mouse

Reactivity: Human, Monkey

Applications: WB, ICC IP,

All Applications: WB: 1:1000
IP: 1:50~200
ICC: 1:50~200

Background: The Ku protein is localized in the nucleus and is composed of subunits referred to as Ku-70 (or p70) and Ku-86 (or p86), which is also known by the synonym Ku-80 (or p80). Ku was first described as an autoantigen to which antibodies were produced in a patient with scleroderma-polymyositis overlap syndrome, and was later found in the sera of patients with other rheumatic diseases. Both subunits of the Ku protein have been cloned, and a number of functions have been proposed for Ku, including cell signaling, DNA replication and transcriptional activation.

Purification and Purity: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: This antibody detects endogenous levels of Ku-70 and does not cross-react with related proteins.

Bioworld Molecular Weight: Predicted band size: 70KDa
Observed band size: 70KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot detection of Ku70 in Hela,A549,COS7 and K562 cell lysates using Ku70 mouse mAb (1:1000 diluted)., Immunocytochemistry staining of HeLa cells fixed with -20℃ Methanol and using anti-Ku70 antibody (dilution 1:200)., Immunoprecipitation analysis of Hela cell lysates using Ku70 mouse mAb.

Alternative Name: X-ray repair cross-complementing protein 6; 5'-deoxyribose-5-phosphate lyase Ku70; 5'-dRP lyase Ku70; 70 kDa subunit of Ku antigen; ATP-dependent DNA helicase 2 subunit 1; ATP-dependent DNA helicase II 70 kDa subunit; CTC box-binding factor 75 kDa subunit; CTC75; CTCBF; DNA repair protein XRCC6; Lupus Ku autoantigen protein p70; Ku70; Thyroid-lupus autoantigen; TLAA; X-ray repair complementing defective repair in; Chinese hamster cells 6; XRCC6; G22P1

Immunogen: Purified recombinant human Ku70 protein fragments expressed in E.coli.

Conjugate: Unconjugated

Modification: Unmodified