Ku70 Monoclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: MB9336-50, MB9336-100
Product: Mouse IgG2a, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P12956
Host: Mouse
Reactivity: Human, Mouse
Applications: WB, IHC
All Applications: WB:1:2,000
IHC:1:100-1:200
Background: Together, Ku70 and Ku80 make up the Ku heterodimer, which binds to DNA double-strand break ends and is required for the non-homologous end joining (NHEJ) pathway of DNA repair. It is also required for V(D)J recombination, which utilizes the NHEJ pathway to promote antigen diversity in the mammalian immune system.In addition to its role in NHEJ, Ku is also required for telomere length maintenance and subtelomeric gene silencing. Ku was originally identified when patients with systemic lupus erythematosus were found to have high levels of autoantibodies to the protein.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: Ku70 monoclonal antibody detects endogenous levels of Ku70 protein.
Bioworld Molecular Weight: 70 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of Ku70 on different cell lysates using anti-Ku70 antibody at 1/2000 dilution.
Positive control:
Lane 1: Hela
Lane 2: HepG2
Lane 3: A431
Lane 4: D3
Lane 5: 293T
Lane 6: A549
Lane 7: COS-1
Lane 8: Human kidney
Lane 9: Human liver
Lane 10: human brain
Lane 11: Human thymus
Lane 12: Human placentae
Lane 13: Mouse kidney
Lane 14: Mouse liver
Lane 15: Mouse thymus
Lane 16: Mouse testis, Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Ku70 antibody. Counter stained with hematoxylin.
Alternative Name: 5'-dRP lyase Ku70, CTC75, CTCBF, Ku70, TLAA, 5''-deoxyribose-5-phosphate lyase Ku70, 5''-dRP lyase Ku70, 70 kDa subunit of Ku antigen, ATP dependent DNA helicase 2 subunit 1, ATP dependent DNA helicase II 70 kDa subunit, ATP-dependent DNA helicase 2 subunit 1, ATP-dependent DNA helicase II 70 kDa subunit, CTC box binding factor 75 kDa subunit, CTC box-binding factor 75 kDa subunit, CTC75, CTCBF, DNA repair protein XRCC6, G22P1, Ku 70, Ku autoantigen p70 subunit, Ku autoantigen, 70kDa, Ku p70, Ku70, Ku70 DNA binding component of DNA-dependent proteinkinase complex (thyroid autoantigen 70 kDa, Kup70, Lupus Ku autoantigen protein p70, ML8, Thyroid autoantigen 70kD (Ku antigen), Thyroid autoantigen, Thyroid lupus autoantigen, Thyroid lupus autoantigen p70, Thyroid-lupus autoantigen, TLAA, X ray repair complementing defective repair in Chinese hamster cells 6, X-ray repair complementing defective repair in Chinese hamster cells 6, X-ray repair cross-complementing protein 6, XRCC 6, Xrcc6, XRCC6,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified