Ku70 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90778-50, BS90778-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P12956(Human)
Host: Rabbit
Reactivity: Human
Applications: WB, IP, ICC/IF IHC FC
All Applications: WB:1:500-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100
IP:1:10-1:50
Background: The Ku protein is localized in the nucleus and is composed of subunits referred to as Ku-70 (p70) and Ku-86 (p86) which is also known by the synonym Ku-80 or (p80). Ku was first described as an autoantigen to which antibodies were produced in a patient with scleroderma polymyositis overlap syndrome, and was later found in the sera of patients with other rheumatic diseases. Both subunits of the Ku protein have been cloned, and a number of functions have been proposed for Ku, including cell signaling, DNA replication and transcriptional activation. Ku is involved in Pol II-directed transcription by virtue of its DNA binding activity, serving as the regulatory component of the DNA-associated protein kinase that phosphorylates Pol II and transcription factor Sp. Ku proteins also activate transcription from the U1 small nuclear RNA and the human transferrin receptor gene promoters. A Ku-related protein designated the enhancer 1 binding factor (E1BF), composed of two subunits, has been identified as a positive regulator of RNA polymerase I transcription initiation.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: Ku70 polyclonal antibody detects endogenous levels of Ku70 protein.
Bioworld Molecular Weight: 70 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of Ku 70 on different cells lysates using anti-Ku 70 antibody at 1/500 dilution.
Positive control:
Line 1: Hela
Line 2: Jurkat, ICC staining Ku 70 in A549 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: 5'-dRP lyase Ku70, CTC75, CTCBF, Ku70, TLAA, XRCC6, 5''-deoxyribose-5-phosphate lyase Ku70, 5''-dRP lyase Ku70, 70 kDa subunit of Ku antigen, ATP dependent DNA helicase 2 subunit 1, ATP dependent DNA helicase II 70 kDa subunit, ATP-dependent DNA helicase 2 subunit 1, ATP-dependent DNA helicase II 70 kDa subunit, CTC box binding factor 75 kDa subunit, CTC box-binding factor 75 kDa subunit, CTC75, CTCBF, DNA repair protein XRCC6, G22P1, Ku 70, Ku autoantigen p70 subunit, Ku autoantigen, 70kDa, Ku p70, Ku70, Ku70 DNA binding component of DNA-dependent proteinkinase complex (thyroid autoantigen 70 kDa, Kup70, Lupus Ku autoantigen protein p70, ML8, Thyroid autoantigen 70kD (Ku antigen), Thyroid autoantigen, Thyroid lupus autoantigen, Thyroid lupus autoantigen p70, Thyroid-lupus autoantigen, TLAA, X ray repair complementing defective repair in Chinese hamster cells 6, X-ray repair complementing defective repair in Chinese hamster cells 6, X-ray repair cross-complementing protein 6, XRCC 6, Xrcc6, XRCC6,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified