L1RE1 Rabbit Monoclonal Antibody-MB66443

L1RE1 Rabbit Monoclonal Antibody

Catalogue Numbers: MB66443-50, MB66443-100

Sizes: 50µl, 100µl

Product: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA.

Swiss-Prot: Q9UN81

Host: Rabbit

Reactivity: Human

Applications: WB, IP

All Applications: WB (1/500 - 1/1000), IP (1/10 - 1/50)

Background: LINE-1, also known as L1, is a non-long terminal repeat (non-LTR) retrotransposon with hundreds of thousands of copies in the human genome. Like all non-LTRs, L1 replicates by target-primed reverse transcription (TPRT). The L1 retrotransposon encodes two proteins critical to this process: ORF1p and ORF2p. ORF2p contributes to endonuclease and reverse transcriptase activity, while ORF1p acts as a nucleic acid chaperone that binds RNA. Many types of cancers have been shown to have L1 insertions within tumor suppressor genes, disrupting their expression and contributing to tumorigenesis.
While normally silenced, ORF1p is expressed in many types of cancers . Recently, it has been found that ORF1p can become phosphorylated and that this event impacts its ability to promote retrotransposition. This creates a novel dynamic to which the host could actively affect L1 replication through various signaling cascades .

Purification and Purity: The antibody was purified by immunogen affinity chromatography.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of L1RE1 protein.

Extra Notes: Western blot analysis of L1RE1 expression in Hela (A), A549 (B) whole cell lysates.

Bioworld Molecular Weight: ~ 40 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: LRE1; LINE-1 retrotransposable element ORF1 protein; L1ORF1p; LINE retrotransposable element 1; LINE1 retrotransposable element 1

Immunogen: Recombinant protein of human LINE-1 ORF1p

Conjugate: Unconjugated

Modification: Unmodified