LAMB3 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS75406-50, BS75406-100
Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q13751
Host: Rabbit
Reactivity: Human, Rat
Applications: WB
All Applications: WB,1:500 - 1:2000
Background: The product encoded by this gene is a laminin that belongs to a family of basement membrane proteins. This protein is a beta subunit laminin, which together with an alpha and a gamma subunit, forms laminin-5. Mutations in this gene cause epidermolysis bullosa junctional Herlitz type, and generalized atrophic benign epidermolysis bullosa, diseases that are characterized by blistering of the skin. Multiple alternatively spliced transcript variants that encode the same protein have been found for this gene.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Unmodification
Extra Notes: Western blot analysis of HeLa, using LAMB3 antibody at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 180s.
Note: For research use only, not for use in diagnostic procedure.
Bioworld Molecular Weight: 130KDa
Alternative Name: LAMB3; AI1A; BM600-125KDA; LAM5; LAMNB1
Immunogen: Recombinant fusion protein of human LAMB3(NP_000219.2).
Conjugate: Unconjugated
Modification: Unmodified