Lamin A/C (5D12) Monoclonal Antibody-MB0181

Lamin A/C (5D12) Monoclonal Antibody

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50µl, 100µl

Catalogue Numbers: MB0181-50, MB0181-100

Swiss-Prot: P02545

Host: Mouse

Lamin A/C (5D12) Monoclonal Antibody-MB0181

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human

Applications: WB, IF

All Applications: WB: 1:1000
IF: 1:50~200

Background: The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Alternative splicing results in multiple transcript variants. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome.

Purification and Purity: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: This antibody detects endogenous levels of Lamin A/C and does not cross-react with related proteins.

Bioworld Molecular Weight: Predicted band size:74, 65KDa
Observed band size:74, 65KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blotDetection of Lamin A/C in MCF7,A549 and Hela cellLysates using Lamin A/C mouse mAb (1:1000 diluted) ., Immunofluorescent analysis of A549 cells fixed with 4% Paraformaldehyde and using anti-Lamin A/C mouse mAb (dilution 1:200) .

Alternative Name: Prelamin-A/C; Lamin-A/C; Lamin A/C; LaminA/C; 70 kDa lamin; Renal carcinoma antigen NY-REN-32; LMNA; LMN1; Lamin A; LaminA; Lamin-A; Lamin C; LaminC; Lamin-C

Immunogen: Purified recombinant human LMNA protein fragments expressed in E.coli.

Modification: Unmodified

Conjugate: Unconjugated