Lamin A(Phospho-Ser22)Polyclonal Antibody-BS65233

Lamin A(Phospho-Ser22)Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS65233-50, BS65233-100

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Swiss-Prot: P02545

Host: Rabbit

Reactivity: Human

Applications: WB, IHC-P, IF/ICC, ELISA

All Applications: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.

Background: lamin A/C(LMNA) Homo sapiens The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Alternative splicing results in multiple transcript variants. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome. [provided by RefSeq, Apr 2012],

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Phospho-Lamin A/C (S392) Polyclonal Antibody detects endogenous levels of Lamin A/C protein only when phosphorylated at S392.

Bioworld Molecular Weight: ~ 74 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Lamin A/C (Phospho-Ser392) Antibody, Immunofluorescence analysis of HeLa cells, using Lamin A/C (Phospho-Ser392) Antibody. The picture on the right is blocked with the phospho peptide., Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Lamin A/C (Phospho-Ser392) Antibody. The picture on the right is blocked with the phospho peptide., Western blot analysis of lysates from HeLa cells, using Lamin A/C (Phospho-Ser392) Antibody. The Lane on the right is blocked with the phospho peptide.

Alternative Name: LMNA; LMN1; Prelamin-A/C

Immunogen: The antiserum was produced against synthesized peptide derived from human Lamin A/C around the phosphorylation site of Ser392. AA range:361-410

Conjugate: Unconjugated

Modification: Phosphorylation