Product Description

Lamin B1 (L75) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS3547-50, BS3547-100

Sizes: 50µl, 100µl

Swiss-Prot: P20700

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: An important part of the nucleus is formed by nuclear lamina. Nuclear lamins form a network of filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished, i.e. A type lamins and B type lamins. The A type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel10, while the B-type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2. The nuclear lamins comprise a unique subclass of the intermediate filament protein family. They share a molecular domain organisation with the other intermediate filament proteins in that they are fibrous molecules that have an aminoterminal globular head, a central rod of alpha helices and a carboxy terminal globular domain. Many biochemical and molecular features of lamins have been studied, but their functions remain still largely undetermined. One of the functions ascribed to the lamina is the maintenance of the structural integrity of the nucleus.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Lamin B1 (L75) polyclonal antibody detects endogenous levels of Lamin B1 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Lamin B1 (L75) pAb at 1:500 dilution
Lane 1: HEK293T whole cell lysate (20ug)
Lane 2: H1792 whole cell lysate (20ug)
Lane 3: The Kidney tissue lysate of Rat (40ug)
Lane 4: The Kidney tissue lysate of Mouse (40ug), Immunohistochemistry (IHC) analyzes of Lamin B1 (L75) pAb in paraffin-embedded human brain tissue.

Bioworld Molecular Weight: ~ 68 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Lamin-B1; LMNB1; LMN2; LMNB

Immunogen: Synthetic peptide, corresponding to amino acids 50-100 of Human Lamin B1.

Conjugate: Unconjugated

Modification: Unmodified