Lamin B1 Monoclonal Antibody-MB66605

Lamin B1 Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB66605-50, MB66605-100

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Swiss-Prot: P20700

Host: Mouse

Reactivity: Human, Mouse, Monkey

Applications: WB, IF/ICC

All Applications: WB (1/500 - 1/1000), IF/ICC (1/50 - 1/100)

Background: Lamins are nuclear membrane structural components that are important in maintaining normal cell functions, such as cell cycle control, DNA replication, and chromatin organization. Lamins have been subdivided into types A and B. Type-A lamins consist of lamin A and C, which arise from alternative splicing of the lamin A gene LMNA. Lamin A and C are cleaved by caspases into large (41-50 kDa) and small (28 kDa) fragments, which can be used as markers for apoptosis. Type-B lamins consist of lamin B1 and B2, encoded by separate genes. Lamin B1 is also cleaved by caspases during apoptosis. Research studies have shown that duplication of the lamin B1 gene LMNB1 is correlated with pathogenesis of the neurological disorder adult-onset leukodystrophy.

Purification and Purity: The antibody was purified by immunogen affinity chromatography.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of Lamin B1 protein.

Bioworld Molecular Weight: ~ 66 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Lamin B1 expression in COS7 (A), Molt4 (B), A549 (C), NIH3T3 (D) whole cell lysates., Immunofluorescent analysis of Lamin B1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.

Alternative Name: LMN2; LMNB; Lamin-B1

Immunogen: Recombinant human Lamin B1 protein.

Conjugate: Unconjugated

Modification: Unmodified