Lamin B1 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90784-50, BS90784-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P20700(Human) P14733(Mouse) P70615(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, ICC/IF, IHC, IP,
All Applications: WB:1:1,000
ICC:1:50-1:200
IHC:1:100-1:500
Background: A unique family of cysteine proteases has been described that differs in sequence, structure and substrate specificity from any previously described protease family. This family, termed Ced-3/ICE, functions as key components of the apoptotic machinery and act to destroy specific target proteins which are critical to cellular longevity. Nuclear lamins are critical to maintaining the integrity of the nuclear envelope and cellular morphology as components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations. Nuclear Lamin B is fragmented as a consequence of apoptosis by an unidentified member of the ICE family.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: Lamin B1 polyclonal antibody detects endogenous levels of Lamin B1 protein.
Bioworld Molecular Weight: 70 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of Lamin B1 on different lysates using anti-Lamin B1 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Mouse liver
Lane 2: Mouse brain, Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Lamin B1 antibody. Counter stained with hematoxylin.
Alternative Name: LMNB1, ADLD, lamin B1, Lamin-B1, LMN, LMN2, LMNB, Lmnb1, LMNB1, MGC111419, OTTHUMP00000159218,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified