Product Description

LAT (D156) Polyclonal Antibody

Catalogue Numbers: BS3757-50, BS3757-100

Sizes: 50µl, 100µl

Alternative Name: Linker for activation of T-cells family member 1; 36 kDa phospho-tyrosine adapter protein; pp36; p36-38; LAT

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O43561 

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IHC

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200

Background: LAT, a transmembrane adaptor protein expressed in T, NK and mast cells, is an important mediator for T cell receptor (TCR) signaling. Upon TCR engagement, activated Zap-70 phosphorylates LAT at multiple conserved tyrosine residues within SH2 binding motifs, exposing these motifs as the docking sites for downstream signaling targets. The phosphorylation of LAT at Tyr171 and 191 enables the binding of Grb2, Gads/SLP-76, PLCgamma1 and PI3 kinase through their SH2 domain and translocates them to the membrane. This process eventually leads to activation of the corresponding signaling pathways.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: LAT (D156) polyclonal antibody detects endogenous levels of LAT protein.

Bioworld Molecular Weight: ~ 36, 38 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of LAT (D156) pAb at 1:500 dilution
Lane 1: K562 whole cell lysate(30ug)
Lane 2: HuT78 whole cell lysate(30ug)
Lane 3: Myla2059 whole cell lysate(30ug)
Lane 4: SP2/0 whole cell lysate(20ug)

Immunogen: Synthetic peptide, corresponding to amino acids 131-180 of Human LAT.

Conjugate: Unconjugated

Modification: Unmodified