LAT (Phospho Tyr255) rabbit pAb - ES20193

LAT (Phospho Tyr255) rabbit pAb

Sizes: 50μL, 100μL

Catalogue Numbers: ES20193-50, ES20193-100

Citations, Manuals and MSDS Available upon request.

Background: function:Required for TCR (T-cell antigen receptor)- and pre-TCR-mediated signaling, both in mature T-cells and during their development. Involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III)-mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Couples activation of these receptors and their associated kinases with distal intracellular events such as mobilization of intracellular calcium stores, PKC activation, MAPK activation or cytoskeletal reorganization through the recruitment of PLCG1, GRB2, GRAP2, and other signaling molecules., miscellaneous:Engagement of killer inhibitory receptors (KIR) disrupts the interaction of PLCG1 with LAT and blocks target cell-induced activation of PLC, maybe by inducing the dephosphorylation of LAT., PTM:Palmitoylation of Cys-26 and Cys-29 is required for raft targeting and efficient phosphorylation., PTM:Phosphorylated on tyrosines by ZAP-70 upon TCR activation, or by SYK upon other immunoreceptor activation; which leads to the recruitment of multiple signaling molecules. Is one of the most prominently tyrosine-phosphorylated proteins detected following TCR engagement., subcellular location:Present in lipid rafts., subunit:When phosphorylated, interacts directly with the PIK3R1 subunit of phosphoinositide 3-kinase and the SH2 domains of GRB2, GRAP, GRAP2, PLCG1 and PLCG2. Interacts indirectly with CBL, SOS, VAV, and LCP2. Interacts with SHB, SKAP2 and CLNK (By similarity). Interacts with FCGR1A., tissue specificity:Expressed in thymus, T-cells, NK cells, mast cells and, at lower levels, in spleen. Present in T-cells but not B-cells (at protein level).,

Alternate Name: Linker for activation of T-cells family member 1 (36 kDa phospho-tyrosine adapter protein; pp36; p36-38)

Source: Rabbit

Applications: WB; ELISA; IHC

Dilution: WB 1:500-2000; IHC-p 1:50-300; ELISA 2000-20000

Reactivity: Human; Mouse; Rat

Immunogen: Synthesized peptide derived from human LAT (Phospho Tyr255)

Storage and Stability: -20°C/1 year

Clonality: Polyclonal

Isotype: IgG

Concentration: 1 mg/ml

Observed Band (KD): 38kD

Human Gene ID: 27040

Human SWISS Prot NO: O43561

Subcellular Location: Cell membrane; Single-pass type III membrane protein. Present in lipid rafts.

Research Use Only