Product Description

LC3 (phospho-Thr50) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: AP4013-50, AP4013-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Two forms of LC3β , the cytosolic LC3-I and the membrane-bound LC3-II, are produced post-translationally. LC3-I is formed by the removal of the C-terminal 22 amino acids from newly synthesized LC3β , followed by the conversion of a fraction of LC3-I into LC3-II. LC3 enhances fibronectin mRNA translation in ductus arteriosus cells through association with 60S ribosomes and binding to an AU-rich element in the 3’ untranslated region of fibronectin mRNA. This facilitates sorting of fibronectin mRNA onto rough endoplasmic reticμlum and translation. MAP LC3β may also be involved in formation of autophagosomal vacuoles. It is expressed primarily in heart, testis, brain and skeletal muscle.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB

Alternate Names: Microtubμle-associated protein 1 light chain 3 beta, MAP1LC3B, MAP1ALC3, LC3-I, LC3-II, LC3I, LC3II, MAP1LC3A

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylation

Immunogen: Synthetic phosphopeptide derived from human LC3 around the phosphorylation site of Threonine 50.

Dilution: WB: 1:500~1:1000

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: LC3 (phospho-Thr50) polyclonal antibody detects endogenous levels of LC3A and LC3B protein only when phosphorylated at Thr50. This antibody also recognizes LC3C when phosphorylated at the corresponding residues.

Molecular Weight: ~ 15 kDa

Pathway: Phosphoinosotode Signaling, Autophagy Signaling,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q9GZQ8/Q9H492/Q9BXW4

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only