Product Description

LC3A (8F5) Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB3283-50, MB3283-100

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Swiss-Prot: Q9H492

Host: Mouse

Reactivity: Human, Rat, Mouse

Applications: WB, IHC-P

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100

Background: Autophagy marker Light Chain 3 (LC3) was originally identified as a subunit of microtubule-associated proteins 1A and 1B (termed MAP1LC3), and subsequently found to contain similarity to the yeast protein Apg8/Aut7/Cvt5 critical for autophagy. Three human LC3 isoforms (LC3A, LC3B, and LC3C) undergo post-translational modifications during autophagy. Cleavage of LC3 at the carboxy terminus immediately following synthesis yields the cytosolic LC3-I form.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG1

Bioworld Molecular Weight: Calculated MW: 14 kDa; Observed MW: 14,16 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of LC3A in Hela lysates, 3T3 lysates, rat Brain lysates using LC3A antibody., Immunohistochemistry analysis of paraffin-embedded Human Heptacarcinoma Tissue using LC3A antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval., Immunohistochemistry analysis of paraffin-embedded Human Prostate Carcinoma Tissue using LC3A antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Alternative Name: Microtubule-associated proteins 1A/1B light chain 3A; Autophagy-related protein LC3 A; Autophagy-related ubiquitin-like modifier LC3 A; MAP1 light chain 3-like protein 1; MAP1A/MAP1B light chain 3 A; MAP1A/MAP1B LC3 A; Microtubule-associated protein 1 light chain 3 alpha

Immunogen: Purified recombinant protein expressed in E.coli.

Conjugate: Unconjugated

Modification: Unmodified