Product Description

LC3A Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB11186-50, MB11186-100

Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Swiss-Prot: Q9H492

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-P, IP,

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IP: 1/20

Background: Autophagy marker Light Chain 3 (LC3) was originally identified as a subunit of microtubule-associated proteins 1A and 1B (termed MAP1LC3), and subsequently found to contain similarity to the yeast protein Apg8/Aut7/Cvt5 critical for autophagy. Three human LC3 isoforms (LC3A, LC3B, and LC3C) undergo post-translational modifications during autophagy. Cleavage of LC3 at the carboxy terminus immediately following synthesis yields the cytosolic LC3-I form.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 14 kDa; Observed MW: 14,16 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of LC3A in 3T3, mouse heart, mouse brain lysates using LC3A antibody., Western blot analysis of LC3A in K562, rat Brain, Hela lysates using LC3A antibody., Immunohistochemistry analysis of paraffin-embedded Human breast cancer using LC3A antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Alternative Name: Microtubule-associated proteins 1A/1B light chain 3A; Autophagy-related protein LC3 A; Autophagy-related ubiquitin-like modifier LC3 A; MAP1 light chain 3-like protein 1; MAP1A/MAP1B light chain 3 A; MAP1A/MAP1B LC3 A; Microtubule-associated protein 1 light chain 3 alpha

Immunogen: A synthetic peptide of human MAP1LC3A

Conjugate: Unconjugated

Modification: Unmodified