Product Description

LC3A Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB12111-50, MB12111-100

Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Swiss-Prot: Q9H492

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IP,

All Applications: WB: 1/500-1/1000 IP: 1/20

Background: Autophagy marker Light Chain 3 (LC3) was originally identified as a subunit of microtubule-associated proteins 1A and 1B (termed MAP1LC3), and subsequently found to contain similarity to the yeast protein Apg8/Aut7/Cvt5 critical for autophagy. Three human LC3 isoforms (LC3A, LC3B, and LC3C) undergo post-translational modifications during autophagy. Cleavage of LC3 at the carboxy terminus immediately following synthesis yields the cytosolic LC3-I form.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 14 kDa; Observed MW: 14,16 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of LC3A in rat brain lysates using LC3A antibody., Western blot analysis of LC3A in A549, MCF-7, 293T, C2C12 lysates using LC3A antibody.

Alternative Name: Microtubule-associated proteins 1A/1B light chain 3A; Autophagy-related protein LC3 A; Autophagy-related ubiquitin-like modifier LC3 A; MAP1 light chain 3-like protein 1; MAP1A/MAP1B light chain 3 A; MAP1A/MAP1B LC3 A; Microtubule-associated protein 1 light chain 3 alpha

Immunogen: A synthetic peptide of human MAP1LC3A

Conjugate: Unconjugated

Modification: Unmodified