LDHA/LDHB Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90793-50, BS90793-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P00338(Human) P07195(Human) P06151(Mouse) P16125(Mouse) P04642(Rat) P42123(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat, zebrafish
Applications: WB, ICC/IF, IHC, IP, , FC
All Applications: WB:1:1,000-1:5,000
ICC:1:50-1:200
IHC:1:200-1:500
FC:1:50-1:100
Background: The lactate dehydrogenase family (LDH) catalyzes the final step of anaerobic glycolysis, the conversion of L-lactate and NAD to pyruvate and NADH. The LDH family consists of three members, LDH-A, LDH-B and LDH-C, all of which form tetramers consisting four subunits. However, each family member displays a specific tissue distribution pattern with LDH-A and LDH-B predominant in several tissues, specifically LDH-A in muscle and LDH-B in heart, while LDH-C expression is confined to the testis and sperm. LDHs function as powerful markers for germ cell tumors. The genes encoding human LDH-A and LDH-C map to chromosome 11, while the human LDH-B gene maps to chromosome 12. Deficiency in the LDH-A gene is linked to exertional myoglobinuria.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: LDHA/LDHB polyclonal antibody detects endogenous levels of LDHA/LDHB protein.
Bioworld Molecular Weight: 37 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of Lactate Dehydrogenase on different lysates using anti-Lactate Dehydrogenase antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: A549
Lane 3: MCF-7, ICC staining Lactate Dehydrogenase in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: LDH-A, LDH-M, LDHA, PIG19, LDH-B, LDH-H, LDHB, Cell proliferation-inducing gene 19 protein, GSD11, L lactate dehydrogenase B chain, L-lactate dehydrogenase A chain, Lactate dehydrogenase A, Lactate dehydrogenase B, Lactate dehydrogenase H chain, Lactate dehydrogenase M, LDH A, LDH B, LDH H, LDH heart subunit, LDH M, LDH muscle subunit, LDH-A, LDH-M, LDH1, ldha, LDHA, LDHBD, LDHM, MS1111, PIG19, Proliferation inducing gene 19, Renal carcinoma antigen NY REN 46, Renal carcinoma antigen NY-REN-59, TRG 5, TRG5,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified