LDL Receptor Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90794-50, BS90794-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P01130(Human) P35951(Mouse)
Host: Rabbit
Reactivity: Human, Mouse
Applications: WB, ICC/IF, IHC, FC
All Applications: WB:1:1,000-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100
Background: LDLR (low density lipoprotein receptor) is a member of the LDL receptor gene family, which includes LDLR, LRP, Megalin, VLDLR, and ApoER2. The LDL receptor family is characterized by a cluster of cysteine-rich class A repeats, epidermal growth factor (EGF)-like repeats, YWTD repeats, and an O-linked sugar domain. The LDL receptor is a cell surface transmembrane protein that mediates the uptake of low density lipoprotein and its degradation in the lysosome, which provides cholesterol to cells. The cytoplasmic domain of the LDL receptor is necessary for the receptor to cluster in coated pits, which promotes the rapid endocytosis of bound LDL. Mutations in LDLR cause the autosomal dominant disease, familial hypercholesterolemia (FH), which promotes premature coronary atherosclerosis.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: LDL Receptor polyclonal antibody detects endogenous levels of LDL Receptor protein.
Bioworld Molecular Weight: 140 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of LDL Receptor on MCF-7 lysates using anti-LDL Receptor antibody at 1/1,000 dilution., ICC staining LDL Receptor in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: LDL receptor, FH, FHC, LDL R, LDL receptor, LDLCQ2, Ldlr, LDLR, Low Density Lipoprotein Receptor, Low density lipoprotein receptor class A domain containing protein 3, Low density lipoprotein receptor familial hypercholesterolemia, Low-density lipoprotein receptor,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified