Product Description

LEF1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BZ17014-50, BZ17014-100

Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Swiss-Prot: Q9UJU2

Host: Rabbit

Reactivity: Human, Rat

Applications: WB, IHC-F, IHC-P, ICC/IF, FC, IP,

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20 FC: 1/50-1/100

Background: Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity).

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 44 kDa; Observed MW: 25-58 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunocytochemistry analysis of LEF1 in Jurkat using LEF1 antibody, and DAPI., Western blot analysis of LEF1 in C6 lysates using LEF1 antibody., Western blot analysis of LEF1 in Jurkat lysates using LEF1 antibody, Immunohistochemistry analysis of paraffin-embedded Human tonsil using LEF1 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Alternative Name: LEF1; Lymphoid enhancer-binding factor 1; LEF-1; T cell-specific transcription factor 1-alpha; TCF1-alpha

Immunogen: A synthetic peptide of human LEF1

Conjugate: Unconjugated

Modification: Unmodified