Product Description

Legumain (LGMN) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS74558-50, BS74558-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q99538

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF/ICC

All Applications: WB,1:1000 - 1:2000 | IHC,1:50 - 1:200 | IF/ICC,1:50 - 1:200

Background: This gene encodes a cysteine protease that has a strict specificity for hydrolysis of asparaginyl bonds. This enzyme may be involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Enzyme activation is triggered by acidic pH and appears to be autocatalytic. Protein expression occurs after monocytes differentiate into dendritic cells. A fully mature, active enzyme is produced following lipopolysaccharide expression in mature dendritic cells. Overexpression of this gene may be associated with the majority of solid tumor types. This gene has a pseudogene on chromosome 13. Several alternatively spliced transcript variants have been described, but the biological validity of only two has been determined. These two variants encode the same isoform.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Bioworld Molecular Weight: 36KDa/55KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Mouse kidney, using Legumain antibody at 1:632 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s., Immunohistochemistry of paraffin-embedded mouse kidney using Legumain Rabbit pAb at dilution of 1:50. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol., Immunofluorescence analysis of C6 cells using Legumain) Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining., Immunofluorescence analysis of HeLa cells using Legumain) Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Alternative Name: LGMN; AEP; LGMN1; PRSC1; legumain

Immunogen: Recombinant fusion protein of human Legumain (Legumain (LGMN))(NP_001008530.1).

Conjugate: Unconjugated

Modification: Unmodified