Product Description

LGALS9 Mouse Monoclonal Antibody

Sizes: 25μl, 100μl

Catalogue Numbers: MB4003-25, MB4003-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Galectins are a family of β-galactose binding proteins that are characterized by an affinity for poly-N-acetyllactosamine-enriched glycoconjugates and a carbohydrate-binding site. Members of the galectin family have been implicated in a variety of biological functions, including cell adhesion, growth regμlation, cytokine production, T-cell apoptosis, and immune responses. Galectin-9 is induced by proinflammatory stimμli, including IFN-γ, TNF-α, and TLR ligands, and regμlates various immune responses through interaction with its ligand TIM-3. Binding of galectin-9 to TIM-3 expressed by Th1 CD4 T cells resμlted in T cell death. On the other hand, galectin-9 treatment of tumor-bearing mice increased the number of IFN-γ-producing TIM-3+ CD8 T cells and TIM-3+ dendritic cells. Transgenic overexpression of either TIM-3 or galectin-9 in mice led to an increase in cells with a myeloid-derived suppressor cell phenotype and inhibition of immune responses. CD44 is also proposed to be a receptor for galectin-9, and interaction of galectin-9 with CD44 expressed by induced regμlatory T (iTreg) cells enhanced the stability of function of iTreg cells. In addition, galectin-9 was recently demonstrated to bind Dectin-1 expressed by pancreatic ductal adenocarcinoma-infiltrating macrophages, resμlting in tolerogenic macrophage reprogramming and suppression of anti-tumor immunity. Increased galectin-9 expression has been observed in several cancer types, including lung, liver, breast, and kidney. Alternative splicing of the galectin-9 transcript leads to several isoforms.

Category: Primary Antibody

Reactivity: Human

Host: Mouse

Applications: IHC, WB

Alternate Names: Galectin-9, Gal-9, Ecalectin, Tumor antigen HOM-HD-21

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodified

Dilution: WB 1:2000, IHC 1:150

Purification: The antibody was affinity-purified from mouse ascites fluids or tissue cμlture supernatant by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: IgG2b

Molecular Weight: ~ 39 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: O00182

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only