LIF Polyclonal Antibody
Catalogue Numbers: BS60463-50, BS60463-100
Sizes: 50µl, 100µl
Alternative Name: Leukemia inhibitory factor; LIF; Differentiation-stimulating factor; D factor; Melanoma-derived LPL inhibitor; MLPLI; INN=Emfilermin; LIF; HILDA
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P15018
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB
All Applications: WB: 1:500~1:1000
Background: Embryonic stem (ES) cells are the focus of much research and represent great therapeutic potential as they can be propagated indefinitely in an undifferentiated state while possessing the ability to differentiate into all embryonic germ layers (endoderm, ectoderm and mesoderm) both in vivo and in vitro. LIF (leukemia inhibitory factor), also known as MLPLI (melanoma-derived LPL inhibitor), HILDA, DIA or CDF, is a 202 amino acid secreted protein and lymphoid factor that participates in the maintenance of ES cell pluripotency by suppressing spontaneous ES cell differentiation. Secreted LIF precursor is further processed into a biologically active glycoprotein. Expressed by a wide variety of cells including activated T lymphocytes, monocytes, mast cells and neuronal cells, LIF is suggested to promote survival and growth of axons in vitro and is involved in immune tolerance at the maternal-fetal interface. LIF may also participate in fat and bone metabolism and regulate epithelial conversion during kidney development.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: LIF polyclonal antibody detects endogenous levels of LIF protein.
Bioworld Molecular Weight: ~ 22 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of LIF polyclonal antibody at 1:500 dilution
Lane 1: Jurkat whole cell lysate
Lane 2: NIH-3T3 whole cell lysate
Lane 3: PC12 whole cell lysate
Immunogen: A synthetic peptide corresponding to residues in Human LIF.
Conjugate: Unconjugated
Modification: Unmodified