Product Description

LIM Kinase 2 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BZ16337-50, BZ16337-100

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Swiss-Prot: P53671

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-P, ICC/IF

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 ICC: 1/100-1/200

Background: Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics (PubMed:10436159, PubMed:11018042). Acts downstream of several Rho family GTPase signal transduction pathways (PubMed:10436159, PubMed:11018042). Involved in astral microtubule organization and mitotic spindle orientation during early stages of mitosis by mediating phosphorylation of TPPP (PubMed:22328514). Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro (PubMed:8537403). Suppresses ciliogenesis via multiple pathways; phosphorylation of CFL1, suppression of directional trafficking of ciliary vesicles to the ciliary base, and by facilitating YAP1 nuclear localization where it acts as a transcriptional corepressor of the TEAD4 target genes AURKA and PLK1 (PubMed:25849865).

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 72 kDa; Observed MW: 72 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of LIM Kinase 2 in HepG2, HUVEC lysates using LIM Kinase 2 antibody., Immunohistochemistry analysis of paraffin-embedded Human heart tissue using LIMK2 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval. Sample with blocking peptide on the right., Immunofluorescence analysis of LIM Kinase 2 in Hela cells using LIMK2 antibody.

Alternative Name: LIM domain kinase 2

Immunogen: Synthetic peptide of human LIMK2

Conjugate: Unconjugated

Modification: Unmodified