LIMK-2 (phospho Thr505) Polyclonal Antibody - RA21137

LIMK-2 (phospho Thr505) Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: RA21137-50, RA21137-100

Citations, Manuals and MSDS Available upon request.

Background: LIM domain kinase 2 (LIMK2) Homo sapiens There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by this gene is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],

Condition: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage Instructions: -20°C/1 year

Recommended dilutions: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.

Alternative Names: LIMK2; LIM domain kinase 2; LIMK-2

Applications: WB; IHC-p; IF/ICC; ELISA

Species Cross-Reactivity: Human; Mouse; Rat

GeneID (Human): 3985

SWISS: P53671

Source: Rabbit

Research Use Only