LIMK-2 Polyclonal Antibody - BT-AP05020

LIMK-2 Polyclonal Antibody

Sizes: 20μL, 50μL, 100μL

Catalogue Numbers: BT-AP05020-20, BT-AP05020-50, BT-AP05020-100

Citations, Manuals and MSDS Available upon request.

Background: There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by LIMK2 is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene.

Research Areas: Signal Transduction

Target Protein: LIMK2

Applications: WB, ELISA

Reactivity: Human, Mouse, Rat

Clonality: Polyclonal

Host: Rabbit

Isotype: IgG

Full Product Name: LIMK-2 Antibody

Immunogen: The antiserum was produced against synthesized peptide derived from human AMPK alpha around the phosphorylation site of Thr172. AA range:140-189

Storage: -20°C for 1 year

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration: 1 mg/ml

Molecular Weight (Da): 72232

UniProt Accession: Human: P53671; Mouse: O54785; Rat: P53670

Synonyms: LIMK2; LIM domain kinase 2; LIMK-2

GeneID: Human: 3985; Mouse: 16886; Rat: 29524

Research Use Only