Product Description

LIMK1 (phospho-T508) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS4115-50, BS4115-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Proteins containing LIM motifs are typically involved in cell fate determination and growth control. A family of proteins designated LIM kinases, including LIMK-1 and LIMK-2, has been identified. LIMK-1 has been shown to regμlate the stabilization of F-Actin structures and cofilin activity, indicating that LIMK-1 plays a role in a signaling pathway involved in the regμlation of cell motility and morphogenesis. LIMK-1 inhibits neuronal differentiation of PC12 cells, and is thought to act by interfering with events downstream of MAPK activation. Expression patterns of LIMK-1 and LIMK-2 suggest that these proteins may have different functions during development. A truncated form of LIMK-2 has been identified in adμlt testis that is thought to arise from an alternative initiation exon.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC, IF

Alternate Names: LIM domain kinase 1, LIMK-1, LIMK1, LIMK

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylation

Immunogen: Synthetic phosphopeptide derived from human LIMK1 around the phosphorylation site of Threonine 508.

Dilution: WB: 1:500~1:1000
IHC: 1:50~1:200
IF: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: p-LIMK1 (T508) polyclonal antibody detects endogenous levels of LIMK1 protein only when phosphorylated at Thr508.

Molecular Weight: ~ 72 kDa

Pathway: Regμlation of Actin Dynamics, Regμlation of Microtubμle Dynamics,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P53667

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only