Product Description

LIN28A Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB66632-50, MB66632-100

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Swiss-Prot: Q9H9Z2

Host: Mouse

Reactivity: Human

Applications: WB, IF/ICC

All Applications: WB (1/500 - 1/1000), IF/ICC (1/50 - 1/100)

Background: LIN28A and LIN28B are conserved, developmentally regulated RNA binding proteins that inhibit the processing and maturation of the let-7 family of miRNAs. The let-7 miRNAs have been implicated in repression of oncogenes such as Ras, Myc, and HMGA2. It has recently been shown that upregulation of LIN28A and LIN28B in primary human tumors and human cancer cell lines is correlated with downregulation of let-7 miRNAs. LIN28 genes are reported to be involved in primordial germ cell development and germ cell malignancy. In addition, allelic variation in LIN28B is associated with regulating the timing of puberty in humans. Overexpression of LIN28A, in conjunction with Oct-4, Sox2, and Nanog, can reprogram human fibroblasts to pluripotent, ES-like cells.

Purification and Purity: The antibody was purified by immunogen affinity chromatography.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of LIN28A protein.

Bioworld Molecular Weight: ~ 25 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of LIN28A expression in NTERA2 (A) whole cell lysates., Immunofluorescent analysis of LIN28A staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Alternative Name: CSDD1; LIN28; ZCCHC1; Protein lin-28 homolog A; Lin-28A; Zinc finger CCHC domain-containing protein 1

Immunogen: Purified recombinant fragment of LIN28 (aa93-209) expressed in E. Coli.

Conjugate: Unconjugated

Modification: Unmodified