Product Description

LKB1 (Phospho-Ser428) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: AP0088-50, AP0088-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: LKB1 (STK11) is a serine/threonine kinase and tumor suppressor that helps control cell structure, apoptosis and energy homeostasis through regμlation of numerous downstream kinases. A cytosolic protein complex comprised of LKB1, putative kinase STRAD, and the MO25 scaffold protein, activates both AMP-activated protein kinase (AMPK) and several AMPK-related kinases. AMPK plays a predominant role as the master regμlator of cellμlar energy homeostasis, controlling downstream effectors that regμlate cell growth and apoptosis in response to cellμlar ATP concentrations. LKB1 appears to be phosphorylated in cells at several sites, including human LKB1 at Ser31/325/428 and Thr189/336/363. Mutation in the corresponding LKB1 gene causes Peutz-Jeghers syndrome (PJS), an autosomal dominant disorder characterized by benign GI tract polyps and dark skin lesions of the mouth, hands, and feet. A variety of other LKB1 gene mutations have been associated with the formation of sporadic cancers in several tissues. Recent evidence suggests that phosphorylation at Ser428 by PKCζ influences the ability of LKB1 to bind and phosphorylate AMPK at Thr172 as well as regμlate apoptosis through PTEN signaling pathway suppression of Akt.

Category: Primary Antibody

Reactivity: Human, Rat

Host: Rabbit

Applications: WB

Alternate Names: Serine/threonine-protein kinase STK11, Liver kinase B1, LKB1, Hlkb1, Renal carcinoma antigen NY-REN-19, STK11, LKB1, PJS

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylation

Immunogen: Synthetic phosphopeptide derived from human LKB1 around the phosphorylation site of Serine 428.

Dilution: WB: 1:1000~1:2000

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: LKB1(Phospho-Ser428) polyclonal antibody detects endogenous levels of LKB1 protein only when phosphorylated at Ser428.

Molecular Weight: ~ 55 kDa

Pathway: Warburg Effect, Translational Contral elF4 and p70 S6 Kinase, Glutamine Metabolism, AMPK Signaling Pathway, MTOR signaling,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q15831

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Research Use Only