LLGL2 Polyclonal Antibody Sizes: 50µl, 100µl Catalogue Numbers: BS77683-50, BS77683-100 Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Swiss-Prot: Q6P1M3 Host: Rabbit Reactivity: Human Applications: WB All Applications: WB,1:500 - 1:2000 Background: The lethal (2) giant larvae protein of Drosophila plays a role in asymmetric cell division, epithelial cell polarity, and cell migration. This human gene encodes a protein similar to lethal (2) giant larvae of Drosophila. In fly, the protein's ability to localize cell fate determinants is regulated by the atypical protein kinase C (aPKC). In human, this protein interacts with aPKC-containing complexes and is cortically localized in mitotic cells. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity: Unmodification Extra Notes: Western blot analysis of extracts of HeLa cells, using LLGL2 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s. Note: For research use only, not for use in diagnostic procedure. Bioworld Molecular Weight: 113kDa Alternative Name: HGL; Hugl-2; LGL2; LLGL2 Immunogen: Recombinant fusion protein of human LLGL2(NP_001026973.1). Conjugate: Unconjugated Modification: Unmodified