Product Description

LRAT Polyclonal Antibody

Catalogue Numbers: BS5783-50, BS5783-100

Sizes: 50µl, 100µl

Alternative Name: Lecithin retinol acyltransferase; Phosphatidylcholine--retinol O-acyltransferase; Phosphatidylcholine-retinol O-acyltransferase

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O95237

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB IF

All Applications: WB: 1:500~1:1000
IF: 1:50~1:200

Background: Lecithin retinol acyltransferase (LRAT) is a membrane bound enzyme that catalyzes the transfer of an acyl group from the sn-1 position of lecithin to vitamin A which generates all-trans-retinyl esters (tREs) in the liver, some extrahepatic tissues, such as the lung, and retinal pigmented epithelium. LRAT can also exchange palmitoyl groups between RPE65, a tRE binding protein essential for vision, and tREs, which is important for the operation of the visual pathway. LRAT is essential for the dietary mobilization, transport, and storage of vitamin A as well as the synthesis of the visual pigment chromophore. LRAT monomers interact in membranes to form homodimers through disulfide bond formation. A loss of LRAT correlates with an early onset severe retinal dystrophy and severe retinyl ester deprivation, while a reduction in LRAT expression may be associated with invasive bladder cancer.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: LRAT polyclonal antibody detects endogenous levels of LRAT protein.

Bioworld Molecular Weight: ~ 25 kDa (Monomer), 50 kDa (Dimer)

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of LRAT polyclonal antibody at 1:500 dilution
Lane 1: HEK293T cell lysate
Lane 2: sp2/0 cell lysate
Lane 3: H9C2 cell lysate

Immunogen: Synthetic peptide, corresponding to the 148-193 of Human LRAT.

Conjugate: Unconjugated

Modification: Unmodified