LRP1 Rabbit Monoclonal Antibody-BS9805M

LRP1 Rabbit Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS9805M-50, BS9805M-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q07954

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF, IHC, IP, , FC

All Applications: WB: 1:1000-1:5000 IHC/ICC/IF: 1:50-1:200
IP: 1:50-1:200 FC: 1:10-1:100

Background: LRP1, also designated LRP and α-2-Macroglobulin receptor, is an endocytic receptor that mediates the uptake of at least 15 ligands, including α-2-Macroglobulin and apoE. LRP1 is cleaved into a membrane subunit and an extracellular subunit, which remain non-covalently associated. Proper folding and trafficking of LRP1 is facilitated by the receptor-associated protein (RAP), a molecular chaperone. The uptake of all known ligands through LRP1 can be blocked by RAP, which induces a conformational change in the receptor that renders it unable to bind ligands. LRP1, which is expressed in brain, liver and lung, is also implicated in Alzheimer’s disease (AD), as the human LRP gene localizes to a potential AD locus on chromosome 12.

Purification and Purity: Protein A affinity purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: This antibody detects endogenous levels of LRP1 and does not cross-react with related proteins.

Bioworld Molecular Weight: ~ 85 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of LRP1 Rabbit mAb at 1:1000 dilution
Lane 1: The liver tissue lysate of Mouse
Lane 2: The brain tissue lysate of Mouse
Lane 3: The lung tissue lysate of Mouse
Lane 4: The liver tissue lysate of Human
Lane 5: HepG2 whole cell lysate
Lane 6: The lung tissue lysate of Human, Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-LRP1 antibody. Counter stained with hematoxylin., ICC staining LRP1 in HUVEC cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS., Flow cytometric analysis of Hela cells with LRP1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor® 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Alternative Name: A2MR; Alpha 2 macroglobulin receptor; alpha 2MR; Alpha-2-macroglobulin receptor; APOER; Apolipoprotein E receptor; APR; CD 91; CD91; CD91 antigen; Low density lipoprotein receptor related protein 1; Low density lipoprotein related protein 1; Low-density lipoprotein receptor-related protein 1 intracellular domain; LRP 1; LRP 515; LRP 85; LRP; LRP ICD; LRP-1; LRP-515; LRP-85; Lrp1; LRP1 protein; LRP1; LRP515; LRP85; LRPICD; MGC88725; TGFBR 5; TGFBR5; Type V tgf beta receptor;

Immunogen: Recombinant antibody.

Conjugate: Unconjugated

Modification: Unmodified