LSD1 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90810-50, BS90810-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: O60341(Human) Q6ZQ88(Mouse)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, ICC IHC
All Applications: WB:1:500-1,000
ICC:1:500-1:2,000
IHC:1:50-1:200
FC:1:50-1:100
Background: Histone methylation regulates chromatin structure and transcription and maintains an epigenetic state of the cell. Histone methylation is dynamically regulated by histone methylases and demethylases. Lysine-specific histone demethylase 1 (LSD1), also designated BHC110, is a flavin-dependent amine oxidase which catalyzes the removal of one or two methyl groups from the methyl-lysine-4 side chain of Histone H3. The LSD1 protein contains a SWIRM domain, a FAD-binding motif and an amine oxidase domain. Association with CoREST, a SANT domain-containing corepressor, positively regulates LSD1. CoREST mediates the demethylation ability of LSD1 and protects it from proteasomal degradation in vivo. PHF21A (also designated BCH80), a PHD domain-containing protein, inhibits activity of LSD1/CoREST mediated de-methylation. The LSD1 protein also co-localizes with the androgen receptor in human prostate tumor cells and in unaffected prostate cells, stimulating androgen-receptor-dependent transcription.
Purification and Purity: Peptide affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: LSD1 polyclonal antibody detects endogenous levels of LSD1 protein.
Bioworld Molecular Weight: 110 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of LSD1 on PC-12 and SiHa cell lysates using anti-LSD1 antibody at 1/1,000 dilution., ICC staining LSD1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: KDM1A, AOF2, KDM1, KIAA0601, LSD1, Amine oxidase (flavin containing) domain 2, Amine oxidase, flavin containing, 2, AOF2, BHC110, BRAF35 HDAC complex protein BHC110, BRAF35-HDAC complex protein BHC110, BRAF35/HDAC complex, 110-kD subunit, CPRF, EC1, FAD binding protein BRAF35 HDAC complex, 110 kDa subunit, Flavin-containing amine oxidase domain-containing protein 2, KDM 1, KDM1, Kdm1a, KDM1A, KIAA0601, LSD 1, LSD1, Lysine (K) specific demethylase 1, Lysine (K) specific demethylase 1A, Lysine demethylase 1A, Lysine specific histone demethylase 1, Lysine specific histone demethylase 1A, Lysine-specific demethylase 1, Lysine-specific demethylase 1A, Lysine-specific histone demethylase 1A,
Immunogen: Peptide
Conjugate: Unconjugated
Modification: Unmodified