LYVE1 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90816-50, BS90816-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q9Y5Y7(Human) Q8BHC0(Mouse) EntrezGene:293186(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, ICC/IF, IHC, FC
All Applications: WB:1:1,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100
Background: Lymphatic vessel endothelial hyaluronan receptor-1 (LYVE-1) is expressed on the cell surface as a protein that is reduced by glycosidase treatment. LYVE-1 is abundant in spleen, lymph node, heart, lung and fetal liver, and is less abundant in appendix, bone marrow, placenta, muscle and adult liver. Expression of LYVE-1 is largely restricted to endothelial cells lining lymphatic vessels and splenic sinusoidal endothelial cells. LYVE-1 binds to both soluble and immobilized hyaluronan (HA) with greater specificity than HCAM. Like HCAM, the LYVE-1 molecule binds both soluble and immobilized HA. However, unlike HCAM, the LYVE-1 molecule co-localizes with HA on the luminal face of the lymph vessel wall and is completely absent from blood vessels. Hence, LYVE-1 is the first lymph-specific HA receptor to be characterized and is a uniquely powerful marker for lymph vessels themselves. LYVE-1 is used as a marker to study tumor lymphangiogenesis, which is an important area of investigation.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: LYVE1 polyclonal antibody detects endogenous levels of LYVE1 protein.
Bioworld Molecular Weight: 35 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of LYVE1 on MCF-7 lysates using anti-LYVE1 antibody at 1/1,000 dilution., ICC staining LYVE1 in A431 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: LYVE-1, CRSBP-1, LYVE1, Cell surface retention sequence-binding protein 1, CRSBP 1, CRSBP-1, CRSBP1, extracellular link domain containing 1, extracellular link domain-containing 1, Extracellular link domain-containing protein 1, HAR, Hyaluronic acid receptor, Lymphatic endothelium specific hyaluronan receptor, lymphatic vessel endothelial hyaluronan receptor 1, Lymphatic vessel endothelial hyaluronic acid receptor 1, LYVE 1, LYVE-1, LYVE1, LYVE1, XLKD1,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified