Product Description

M-cadherin (A108) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS2220-50, BS2220-100

Sizes: 50µl, 100µl

Swiss-Prot: P55291

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: Cadherins are a multigene family of Ca++-dependent cell adhesion molecules. They are transmembrane glycoproteins consisting of an extracellular domain, which mediates Ca++-dependent intercellular adhesion by homophilic interactions, a transmembrane region and a cytoplasmic domain. The extracellular domain is divided into a series of subdomains designated EC1-EC5. Homolgies between different members of the cadherin family are most prominent in the cytoplasmic domain and in EC1 and EC2 and much less so in EC5 of the extracellular domain and in the transmembrane region. The binding properties and specificities of the adhesive function are located in the N-terminal part of the molecules. Four members of the cadherin family have been identified and molecularly cloned from mammalian cells. These include the neuronal (N), epithelial (E), placental (P) and muscle (M) cadherins. M-cadherin is not found in fibroblasts but is expressed at low level in myoblasts and is upregulated following induction of myotube formation, suggesting a specific function in skeletal muscle cell differentiation.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: M-cadherin (A108) polyclonal antibody detects endogenous levels of M-cadherin protein..

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of M-cadherin (A108) Antibody at 1:500 dilution
Lane 1: MG63 whole cell lysate (30ug)
Lane 2: U-87MG whole cell lysate (40ug)
Lane 3: The Brain tissue lysate of Mouse (40ug)
Lane 4: The Brain tissue lysate of Rat (40ug), Immunohistochemistry (IHC) analyzes of M-cadherin (A108) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.

Bioworld Molecular Weight: ~ 91 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Cadherin-15; Muscle cadherin; M-cadherin; Cadherin-14; CDH15; CDH14; CDH3

Immunogen: Synthetic peptide, corresponding to amino acids 80-130 of Human M-cadherin.

Conjugate: Unconjugated

Modification: Unmodified