Product Description

M1-linkage Specific Polyubiquitin Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS79341-50, BS79341-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, DB

All Applications: WB,1:500 - 1:2000 | DB,1:500 - 1:2000

Background: Ubiquitination,onetypeofthemostcommonpost-translationalmodification,mediatestheregulationofproteinhomeostasisinvivo. Substrate proteins can be modified with single ubiquitin moieties or with polymeric ubiquitin chains. Within polyubiquitin chains, ubiquitin can form eight different linkage types, using one of seven internal lysine residues (K6, K11, K27, K29, K33, K48, K63) or methionine at position 1 (M1).Here we focus on a distinct type of ubiquitination that is characterized by an inter-ubiquitin linkage through the N-terminal methionine, called M1-linked or linear ubiquitination. Formation, recognition, and disassembly of linear ubiquitin chains are highly specific processes that are implicated in immune signaling, cell death regulation and protein quality control. Consistent with their role in influencing signaling events, linear ubiquitin chains are formed in a transient and spatially regulated manner, making their detection and quantification challenging.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Other Modified

Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human M1-linkage Specific Polyubiquitin(NP_066289.3/NP_061828.1).

Conjugate: Unconjugated

Modification: Other Modified

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using M1-linkage Specific Polyubiquitin antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s., Dot-blot analysis of all sorts of peptides using M1-linkage Specific Polyubiquitin antibody at 1:1000 dilution.