macroH2A.1 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90817-50, BS90817-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: O75367(Human) Q9QZQ8(Mouse)
Host: Rabbit
Reactivity: Human, Mouse
Applications: WB, IHC FC
All Applications: WB:1:500
IHC:1:50-1:200
FC:1:50-1:100
Background: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Involved in stable X chromosome inactivation. Inhibits the binding of transcription factors, including NF-kappa-B, and interferes with the activity of remodeling SWI/SNF complexes. Inhibits histone acetylation by EP300 and recruits class I HDACs, which induces a hypoacetylated state of chromatin.
Purification and Purity: Protein affinity purified.
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Specificity: macroH2A.1 polyclonal antibody detects endogenous levels of macroH2A.1 protein.
Bioworld Molecular Weight: 40 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of macroH2A.1 on different lysates using anti-macroH2A.1 antibody at 1/500 dilution.
Positive control:
Lane 1: mouse pancreas
Lane 2: SiHa
Lane 3: PC-3M, Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-macroH2A.1 antibody. Counter stained with hematoxylin.
Alternative Name: Histone macroH2A1, mH2A1, H2A/y, H2AFY, MACROH2A1, Core histone macro h2a.1, Core histone macro-H2A.1, H2A histone family member Y, H2A.y, H2A/y, H2AF12M, H2AFJ, H2afy, H2AY, Histone H2A.Y, Histone macroH2A1, Histone macroH2A1.1, Histone macroH2A1.2, Macroh2a1, MACROH2A1.1, MacroH2A1.2, Medulloblastoma antigen MU MB 50.205, Medulloblastoma antigen MU-MB-50.205, mH2a, mH2A1,
Immunogen: Recombinant protein within human macroH2A.1 aa 200-400.
Conjugate: Unconjugated
Modification: Unmodified