Product Description

macroH2A.1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90818-50, BS90818-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O75367(Human) Q9QZQ8(Mouse)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF, IHC

All Applications: WB:1:500-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200

Background: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Involved in stable X chromosome inactivation. Inhibits the binding of transcription factors and interferes with the activity of remodeling SWI/SNF complexes. Inhibits histone acetylation by EP300 and recruits class I HDACs, which induces an hypoacetylated state of chromatin. In addition, isoform 1, but not isoform 2, binds ADP-ribose and O-acetyl-ADP-ribose, and may be involved in ADP-ribose-mediated chromatin modulation.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: macroH2A.1 polyclonal antibody detects endogenous levels of macroH2A.1 protein.

Bioworld Molecular Weight: 40 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of macroH2A.1 on different lysates using anti-macroH2A.1 antibody at 1/500 dilution.
Positive control:
Lane 1: Hela
Lane 2: Mouse placenta tissue
Lane 3: MCF-7, ICC staining macroH2A.1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: Histone macroH2A1, mH2A1, H2A/y, H2AFY, MACROH2A1, Core histone macro h2a.1, Core histone macro-H2A.1, H2A histone family member Y, H2A.y, H2A/y, H2AF12M, H2AFJ, H2afy, H2AY, Histone H2A.Y, Histone macroH2A1, Histone macroH2A1.1, Histone macroH2A1.2, Macroh2a1, MACROH2A1.1, MacroH2A1.2, Medulloblastoma antigen MU MB 50.205, Medulloblastoma antigen MU-MB-50.205, mH2a, mH2A1,

Immunogen: Recombinant protein

Conjugate: Unconjugated

Modification: Unmodified