Product Description

MAD2/MAD2L1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS76766-50, BS76766-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q13257

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: IHC, IF/ICC

All Applications: IHC,1:50 - 1:200 | IF/ICC,1:50 - 1:200

Background: MAD2L1 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is related to the MAD2L2 gene located on chromosome 1. A MAD2 pseudogene has been mapped to chromosome 14.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Immunogen: Recombinant fusion protein of human MAD2/MAD2/MAD2L1(NP_002349.1).

Conjugate: Unconjugated

Modification: Unmodified

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunohistochemistry of paraffin-embedded mouse testis using MAD2/MAD2/MAD2L1 antibody at dilution of 1:100.Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol., Immunofluorescence analysis of NIH-3T3 cells using MAD2/MAD2/MAD2L1 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.