MAD2 rabbit pAb - ES2738

MAD2 rabbit pAb

Sizes: 50μL, 100μL

Catalogue Numbers: ES2738-50, ES2738-100

Citations, Manuals and MSDS Available upon request.

Background: MAD2L1 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is related to the MAD2L2 gene located on chromosome 1. A MAD2 pseudogene has been mapped to chromosome 14. [provided by RefSeq, Jul 2008],

Alternate Name: MAD2L1; MAD2; Mitotic spindle assembly checkpoint protein MAD2A; HsMAD2; Mitotic arrest deficient 2-like protein 1; MAD2-like protein 1

Source: Rabbit

Applications: WB; IP; ELISA

Dilution: Western Blot: 1/500 - 1/2000. IP 1:200-500 ELISA: 1/5000. Not yet tested in other applications.

Reactivity: Human; Rat; Mouse;

Immunogen: The antiserum was produced against synthesized peptide derived from human MAD2L1. AA range:91-140

Storage and Stability: -20°C/1 year

Clonality: Polyclonal

Isotype: IgG

Concentration: 1 mg/ml

Observed Band (KD): 25kD

Human Gene ID: 4085

Human SWISS Prot NO: Q13257

Subcellular Location: Nucleus. Chromosome, centromere, kinetochore. Cytoplasm. Cytoplasm, cytoskeleton, spindle pole. Recruited by MAD1L1 to unattached kinetochores (Probable). Recruited to the nuclear pore complex by TPR during interphase. Recruited to kinetochores in late prometaphase after BUB1, CENPF, BUB1B and CENPE. Kinetochore association requires the presence of NEK2. Kinetochore association is repressed by UBD. Sequestered to the cytoplasm upon interaction with isoform 3 of MAD1L1 (PubMed:19010891).

Research Use Only