Product Description

MAD2B/MAD2L2 Polyclonal Antibody

Sizes: 50 µl, 100 µl

Catalogue Numbers: BS71352-50, BS71352-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9UI95

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: The protein encoded by this gene is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. The encoded protein, which is similar to MAD2L1, is capable of interacting with ADAM9, ADAM15, REV1, and REV3 proteins.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using MAD2B/MAD2B/MAD2L2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.

Bioworld Molecular Weight: 25kDa

Specificity: Polyclonal Antibodies

Alternative Name: MAD2L2; FANCV; MAD2B; POLZ2; REV7

Immunogen: Recombinant fusion protein of human MAD2B/MAD2B/MAD2L2(NP_006332.3).

Conjugate: Unconjugated

Modification: Unmodified