MAGEC2 Polyclonal Antibody-BS5956

MAGEC2 Polyclonal Antibody

Catalogue Numbers: BS5956-50, BS5956-100

Sizes: 50µl, 100µl

Alternative Name: Melanoma-associated antigen C2; Cancer/testis antigen 10; CT10; Hepatocellular carcinoma-associated antigen 587; MAGE-C2 antigen; MAGE-E1 antigen; MAGE-C2; HCA587; MAGEE1

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9UBF1

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: MAGE-C2/C3 (Melanoma-associated antigen C2, Hepatocellular carcinoma-associated antigen 587) is a 373 amino acid protein encoded by the human gene MAGEC2. Genes of the MAGE family direct the expression of tumor antigens that are recognized on human melanomas by autologous cytolytic T lymphocytes. Among tumor tissues, MAGE-C2/C3 is frequently expressed in seminomas, melanomas, and bladder transitional cell carcinomas. It is also expressed in a significant fraction of head and neck carcinomas, breast carcinomas, nonsmall-cell lung carcinomas, and sarcomas. In hepatocellular carcinoma, there is a inverse correlation between tumor differentiation and protein expression, i.e. the lower the differentiation, the higher percentage of expression. MAGE-C2/C3 is not expressed in normal tissues, except in germ cells in the seminiferous tubules and in Purkinje cells of the cerebellum. It is strongly expressed in spermatogonia and primary spermatocytes, however, at later stages of maturation, expression gradually decreases to become undetectable in mature spermatids.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: MAGEC2 polyclonal antibody detects endogenous levels of MAGEC2 protein.

Bioworld Molecular Weight: ~ 41 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of MAGEC2 polyclonal antibody at 1:500 dilution
Lane 1: HEK293T cell lysate
Lane 2: NIH-3T3 cell lysate
Lane 3: PC12 cell lysate

Immunogen: Synthetic peptide, corresponding to amino acids 237-282 of Human MAGEC2.

Conjugate: Unconjugated

Modification: Unmodified