Product Description

MAGED1/NRAGE Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS6145-50, BS6145-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9Y5V3

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

All Applications: WB,1:500 - 1:2000 | IHC,1:50 - 1:200

Background: This gene is a member of the melanoma antigen gene (MAGE) family. Most of the genes of this family encode tumor specific antigens that are not expressed in normal adult tissues except testis. Although the protein encoded by this gene shares strong homology with members of the MAGE family, it is expressed in almost all normal adult tissues. This gene has been demonstrated to be involved in the p75 neurotrophin receptor mediated programmed cell death pathway. Three transcript variants encoding two different isoforms have been found for this gene.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 86KDa/97KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of A-549 cells, using MAGED1/NRAGE Rabbit pAb antibody at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s., Immunohistochemistry of paraffin-embedded human colon carcinoma using MAGED1/NRAGE Rabbit pAb at dilution of 1:500. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded human placenta using MAGED1/NRAGE Rabbit pAb at dilution of 1:500. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded human tonsil using MAGED1/NRAGE Rabbit pAb at dilution of 1:500. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Alternative Name: MAGED1; DLXIN-1; NRAGE

Immunogen: Recombinant fusion protein of human MAGED1/NRAGE(NP_001005333.1).

Conjugate: Unconjugated

Modification: Unmodified